Vol. 9, Issue 1, Part L (2024)

Author(s): Manju Gari, Soumen Choudhury, Amit Shukla and Satish K Garg

Abstract: The present research aimed to explore the impact of sepsis on hematological changes in mice with pre-existing diabetes. Type 1 diabetes was induced by administering streptozotocin at 65 mg/kg body weight via intraperitoneal route for five consecutive days. Sepsis was induced through caecal ligation and puncture. To create a diabeto-septic condition, sepsis was induced after confirming diabetic conditions (8 weeks after the first streptozotocin dose). Blood samples were collected from all groups for hematological parameter assessment. Sepsis led to a significant increase in hemoglobin levels, accompanied by a decrease in other hematological indices, including total erythrocyte count, total leukocyte count, platelet count, hematocrit, granulocytes, monocytes, and lymphocytes. Diabetic mice showed significant reduction in total leukocyte count and lymphocytes, an increase in granulocytes and platelets, with no significant changes in other hematological indices compared to the healthy control. In diabeto-septic mice, hemoglobin levels, total erythrocyte counts, and hematocrit values were nearly comparable to the healthy control, sham-operated, and diabetes groups. However, total leukocyte count and lymphocytes values were found to be intermediate between diabetes and septic mice, but not significantly different from these groups in sepsis in pre-existing diabetic mice. Additionally, monocyte count was significantly lower in diabeto-sepsis than in diabetes, showing significant higher level from sepsis. Granulocytes were significantly decreased in diabeto-sepsis compared to diabetes and were almost comparable to sepsis. Interestingly, sepsis in pre-existing diabetes decreased platelet count to almost normal levels. In conclusion, the coexistence of sepsis and diabetes appears to alter hematological alterations caused by these two conditions individually. However, further studies are necessary to comprehensively understand these interactions.