Vol. 9, Issue 1, Part L (2024)

In subclinical mastitis Staphylococcus aureus (S. aureus) is a prominent bacterium. Resistance to antibiotics is a serious concern when confronted with these kind of infectious agent. In our investigation, 41 of 84 milk samples confirmed positive for bacterial culture and genotypically confirmation done by targeting 23S rRNA. In our investigation, the best-performing effective antibiotics for S. aureus comprised chloramphenicol (87.80%) along with tetracycline (78.04%) afterwards methicillin, and ceftriaxone (75.60%), while the rest of the antibiotics had lesser efficacy. S. aureus had the greatest resistance toward both antibiotics including penicillin-G and Ampicillin (100% each). This study highlights diversified antimicrobial resistance of S. aureus against wide variety of antibiotics. Routine antibiotic resistance surveillance is required to address this issue.