Vol. 9, Issue 1, Part L (2024)

Author(s): Suman Verma, Sanjay Kumawat and Mayank Rawat

Abstract: Brucellosis is the worldwide commonest bacterial re-emerging anthropozoonosis that create a serious public health issue along with significant economic burden on animal industries and poor rural communities. Despite being endemic in many developing countries, this devastating multi-organ disease is often neglected remains as under-diagnosed and under-reported. The economic loses occurs in terms of abortions, temporary infertility and weak offspring and is a major impediment for trade and export. The complete eradication of this disease would be unpractical due to wide host range that includes humans as well as domestic and wild type mammals. However, the strategy of “test and segregation” in conjunction with vaccination is perhaps the only method which is practical and feasible in most developing countries. Public awareness about its zoonotic importance is also a key point to throw out this drastic disease from many endemic areas. Enzyme Linked Immunosorbent assay is the most common assay that use catalytic properties of enzymes to detect and quantify the immunologic interactions. In our research study, we have raised the anti- B. abortus murine serum and immune response in terms of IgM and IgG titers was quantified by Indirect Enzyme Linked Immunosorbent assay on various days post immunization. There was a gradual increase in IgM titer from 2^nd week which peaked at 4^th week and then declined from 5^th week. A constant increase in IgG titer was observed which peaked at 5^th week. Hence, as expected in any post immunization antibody titer the same classical immune response was observed in our study too.