Vol. 9, Issue 1, Part H (2024)

Author(s): Nikhil Pal Bajia, Sumit Prakash Yadav, Anand Kumar, Krishna Kumar Sharma, Shobha Burdak, Pradeep Makawana and Rahul Kumar

Abstract: Ethanolic extract of Giloy (Tinospora cordifolia) was supplemented at concentrations of 100 µg/ml, 300 µg/ml, 500 µg/ml, 700 µg/ml to ovine semen extender in order to study the effect of antioxidant potential on sperm abnormality parameter of Magra ram semen during cooled storage at 4 °C. The basic extender was Tris egg yolk citrate fructose extender (TEYCF) which is supplemented with different concentrations of Giloy extract. Results revealed that, the mean percentage of total sperm abnormality was significantly (p<0.05) lower in 300 µg/ml and 500 µg/ml Giloy extract supplemented group as compare to control after 72 hrs of preservation. After 96 hrs of preservation, the mean percentage of total sperm abnormality was significantly (p<0.05) lower in TC300 and TC100 groups as compared to control. In conclusion, this study provides a new data about positive effect on sperm abnormality parameter of Magra ram semen during cooled storage at 4 °C, after supplementation of the ethanolic extract of Giloy to the extender.

DOI: 10.22271/veterinary.2024.v9.i1h.981