Vol. 9, Issue 1, Part G (2024)

Author(s): Munesh Kumar Pushp, Rohit Kumar Tiwari and Dilip Singh Meena

Abstract: Present study was conducted to compare the efficacy of Ovsynch and double synch protocols on postpartum cyclic and anoestrus buffaloes during the October-January month. Buffaloes belonging farmers in nearby dairy farm and local region of Bharatpur district. 30 buffaloes with parity of 2^nd to 5^th were selected to induce synchronized estrus/estrus using ovsynch and double synch protocols. These animals were randomly divided into two groups. Group I buffaloes (n=15) were treated with Ovsynch protocol on day0 and day 9, 10μg Buserelin acetate), day 7, 500μg cloprostenol sodium (PGF2α) i/m and Group II: In double synch protocol (n=15), buffaloes were treated with 500μg cloprostenol sodium (PGF2α) on Day 0, 10μg Buserelin acetate (GnRH) on Day 2, a second PGF2α on Day 9, and a second GnRH on Day 11. Timed artificial insemination (TAI) was carried out 16-20 hours following the second GnRH in both treatment groups. Buffaloes were routinely observed through rectal examination. Samples of cervico-vaginal mucous were taken directly from the animal's cervix by using cytobrush at the time of artificial insemination. Under a microscope, the cervical mucus of buffaloes was examined for either a typical or atypical fern pattern. A higher chance of conception has been observed in animals with typical fern patterns. Visible behavioral changes and estrus signs i.e. bellowing, mounting, quality and consistency of cervical mucus discharge, pinkish vaginal mucosa and duration of estrus was observed in both groups of buffaloes. During the breeding season, there was a greater estrus response (100%) in double-synch treated buffaloes compared to ovsynch protocol (86.66%) in both farm and field conditions. Behavioral changes and estrus signs were observed in 26.66% (4/15) and 40% (6/15) of buffaloes in Group I and Group II respectively. Group II had a greater conception rate of 60% (9/15) than Group I had a lower rate of 33.3% (5/15). Our findings corroborate and bolster earlier reports that the Double synch treatment increases the Conception rates in postpartum cyclical and anoestrus buffaloes. Additionally, our data show that the Double synch approach increases conception rates. These data thus imply that, independent of the period of the estrous cycle, the Double synch protocol can be employed to achieve satisfactory conception rates following TAI in both anestrous and cycling in buffaloes.