Vol. 9, Issue 1, Part E (2024)

T cells have an important immunoregulatory and effector function through cytokine release. They are involved in the responses to Gram-negative bacterium. In this study, the status of T cell-dependent cytokine gene expressions in the acute phase (3-day post-infection and 5-day post-infection) of heat inactivated pseudomonas culture and live attenuated culture with adjuvant given in SPF chicken of seven-day age was evaluated. The birds were divided into four groups, each having six birds. Each group of birds was inoculated with the prescribed dose of pseudomonas vaccine canditure at 7 days of age. Blood was collected before inoculation (uninfected), at the 3^rd and 5^th day post-inoculation. Presence of pseudomonas culture in peripheral blood confirmed by real-time reverse-transcription PCR assay and quantitation of cytokine was performed in peripheral blood by real time PCR assay. It was observed that the infection with live attenuated pseudomonas culture modulates cytokine expression in order to elicit antibacterial immune responses with respect to killed culture. Live attenuated pseudomonas culture markedly up-regulate IL-4, IL-2 whereas prolonged up-regulation by IFN-γs genes.