Vol. 9, Issue 1, Part C (2024)

Author(s): Vijay Kumar Yogi, Pankaj Kumar Thanvi, Ashok Dangi, Pura Ram, Raj Kumar Siyag, Mahendra Kumar Saini, Priyanka and Sanwar Mal

Abstract: Ten rectums from recently slaughtered adult pigs were used in the current experiment. The tunica muscularis and lamina muscularis responded positively to the McManus PAS stain for glycogen, while the lining epithelium and tunica submucosa did not. Following treatment with saliva, all layers of the rectum for McManus demonstrated PAS-negative reactions for glycogen and mucopolysaccharide. The lining epithelium stained with PAS-Alcian blue pH-2.5 responded strongly positively to the sulphated mucosubstance, however, it reacted only moderately and negatively to PAS-Alcian blue pH-1.0 and pH-0.4, respectively. The keratinized stratified squamous epithelium showed a positive reaction for pre-keratin and keratin but simple columnar epithelium showed a negative reaction for pre-keratin and keratin in Dane’s stain.