Vol. 8, Special Issue 5, Part B (2023)
Characterization of Dichelobacter nodosus isolates by RAPD and SDS-PAGE
Author(s): Vijayalakshmi S, Sreenivasulu D and Rani Prameela D
Abstract: Ovine Footrot is a hoof disease manifested in the form of inter digital lesions of foot of sheep. Regularly in Andhra Pradesh, India the outbreaks of footrot is being reported in sheep causing severe economic loss to the sheep husbandry. Dichelobacter nodosus, an obligatory anaerobic, gram negative bacteria is the principal causative agent. On characterization of D. nodosus strains isolated in our study by RAPD analysis has shown the presence of 400 bp band common to virulent and intermediate strains and the same band is not seen in benign strains of D. nodosus. Similarly, on SDS-PAGE analysis, different protein band profile pattern was observed in different serogroups. ‘I’ and ‘A’ serogroups have common protein banding pattern. ‘C’ Serogroup revealed a different protein band pattern with an extra band at 120KDa. No protein band could be identified which can differentiate virulence of the strains.
How to cite this article:
Vijayalakshmi S, Sreenivasulu D, Rani Prameela D. Characterization of Dichelobacter nodosus isolates by RAPD and SDS-PAGE. Int J Vet Sci Anim Husbandry 2023;8(5S):97-99.