Theileriosis is caused by tick-borne protozoan parasite Theileria annulata causing high morbidity and mortality in cattle. During the period of study, the clinical signs, haematological parameters, biochemical parameters and therapeutic management was evaluated. Various clinical signs recorded in cows affected with Theleriosis were Inappetance, Pyrexia, superficial lymph nodes enlargement, anemia, and respiratory distress. Giemsa stained Blood smear examination revealed various trophozoitic stages of Theleria annulata in the erythrocytes. Hemato-biochemical studies revealed a significant reduction in Hemoglobin, Total erythrocyte count, Packed cell volume, along with significant decrease in leucocytes and neutrophils in the affected animals. Biochemical parameters revealed a significant elevation in ALT and AST with non-significant increase in BUN, Creatinine along with a decrease in Total protein and albumin. Theleriosis affected animals were treated with Buparvaquone @ 2.5 mg/kg b. wt. intramuscularly along with supportive therapy. Following therapy, improvement in the clinical signs and haemato biochemical parameters were recorded.