Vol. 8, Issue 5, Part A (2023)

The present study was planned to evaluate the prophylactic efficacy of Coriandrum sativum (CS) and Murraya koenigii (MK) on adenine-induced chronic kidney disease in rats. The present experiment was conducted on 36 male Sprague-Dawley rats. In order to assess the prophylactic efficacy of bi-herbal aqueous and alcoholic extracts Coriandrum sativum (CS) and Murraya koenigii (MK), were mixed in a 1:1.5 ratio after determined by in-vitro nucleation assay. The rats were randomly divided into six different groups, each group contains six rats. Chronic kidney disease (CKD) was induced in the group II, III, IV, V and VI by adenine @ 200 mg/kg b.wt. daily once through the intragastric route for 28 days. Group I served as control and was given standard pelleted diet. Group II served as adenine control and was given adenine (200 mg/kg b.wt., orally) for 28 days. Groups III and IV received bi-herbal aqueous extracts of CS and MK @ 250 and 500 mg/kg b.wt., for 28 days respectively. Groups V and VI received bi-herbal alcoholic extracts @ 250 and 500 mg/kg b.wt., for 28 days respectively. The blood was collected on 28th day of experiment to analyse the haemato-biochemical parameters. Urine samples were collected by using metabolic cages on 28th day and analyzed for different qualitative and biochemical parameters. The experimental rats were studied for ultrasonographic, gross and histopathological changes in kidneys of different groups. Bi-herbal aqueous and alcoholic extracts treated rats (groups III, IV, V and VI) produced significant improvement in mean body weight and feed consumption as compared to adenine control rats (group-II). The administration of bi-herbal aqueous and alcoholic extracts of CS and MK leaves, along with adenine in prophylactic groups revealed significant improvement on haemato-biochemical and urine parameters. Results of ultrasonographic and histopathological examination of kidney tissues in prophylactic groups was well supported and prevent alterations in kidney associated changes in adenine-induced CKD in rats. Result of the present study showed that the bi-herbal aqueous extracts of CS and MK leaves at dosage of 250 mg/kg b.wt., while bi-herbal alcoholic extracts at dosage of 500 mg/kg b.wt. showed greater efficacy among all prophylactic study groups. Results of the present study showed that aqueous as well as alcoholic bi-herbal extracts of Coriandrum sativum and Murraya koenigii leaves revealed prophylactic efficacy against adenine induced CKD in rats.