Vol. 8, Issue 4, Part G (2023)

Inflammation of udder by microbial infection is one of the leading economic disease in dairy sector. Most of the pathogens especially Pseudomonas aeruginosa are refractory to the antibiotic theraphy. In the present research, a total of 87 milk samples (Mastitis, M=32 and healthy milk, N=55) were collected from surrounding areas of Krishna District and Buffalo Research Station, Venkataramannagudem of West Godavari District, Andhra Pradesh. The samples were analyzed for microbial isolation and molecular characterization of Pseudomonas aeruginosa. On cultural isolation and molecular analysis, mastitis infected milk detected Pseudomonas aeruginosa in four samples and the pathogen was also detected in three healthy milk samples suggesting that Pseudomonas aeruginosa is responsible for sub-clinical mastitis infection in the herd.