Vol. 8, Issue 4, Part F (2023)

The present study is carried out on isolation of Mycoplasma species from respiratory infections of sheep and Goats because Mycoplasma is also one of the major etiological agent in the production of respiratory infections which produces economic losses to small-scale farmers. In the present study a total of 188 Mycoplasma suspected samples, 95 (44 nasal swabs, 49 lung tissues and 2 pleural fluids) from sheep and 93 (56 nasal swabs, 33 lung tissues and 4 pleural fluids) from goats were collected. Isolation carried out on PPLO broth and PPLO agar. Positive samples produce color change in broth from brown to yellow and production of two different types of colonies on agar. One type is typical fried egg colony and other type is nipple-like with prominent tip. Conformation also done by PCR targeting Mycoplasma genus yielding 280 bp product size.