Vol. 8, Issue 4, Part E (2023)

In sheep, exogenous hormones are utilized in an accelerated lambing system for induction of estrous synchronization and to enhance cyclicity in anestrum which will assist in helping the system to produce lamb continuously throughout the year. To initiate the hormonal events of estrous synchronization, therapy approaches may include gonadotropin-releasing hormone, also known as GnRH and Nano GnRH. Exogenous progesterone (P4) or prostaglandin (PGF2α) administration modifies the length of the estrous cycle by either extending it or reducing it. Here we aim to investigate the effect of various estrous synchronization procedures in Kilakarsal ewe’s reproductive cycles during the off-season. Blood samples were collected with jugular venepuncture on the following days, on CIDR insertion, CIDR removal, estrum, on the day of mating and on the 70th day of pregnancy. Serum samples were analyzed using an ELISA kit (RIA) and a direct immunoenzymatic method to figure out the amount of estradiol. The findings revealed that Kilakarsal ewes exhibited higher oestradiol levels in estrum in all the experimental groups, ranging between 13.780.17 to 15.500.43 ng/mL. The investigation of the results revealed the use of the chitosan nano- conjugated GnRH regimen for ES in Kilakarsal sheep had actual advantages. The Results effectively proved the real advantages of the Kilakarsal ewes' ES method utilizing chitosan Nano conjugated GnRH.