Vol. 8, Issue 4, Part D (2023)

Estrus synchronization program results in effective synchronization of stage of the estrous cycle which is associated with a relatively higher conception if combined with fixed time insemination in buffaloes. A total number of 18 healthy pluriparous buffaloes at 60 days postpartum were selected for the study and equally divided into three groups viz., Group I, II, and III. Group I buffalo were inserted with PIVD intravaginally for 9 days and PIVD was removed on 9^th day and 10 µg of GnRH was administered during artificial insemination (AI) was performed 48 hours after withdrawal of PIVD. Group II buffaloes were inserted with PIVD intravaginally for 9 days and PIVD was removed on 9^th day and 2000 IU of hCG 25 mg of PGF2α was administered intramuscularly one day prior to the removal of PIVD. Group III buffaloes were kept as control and artificially inseminated during natural estrus. The conception rate after first and second service and overall observed in this study were 33.33, 16.67 and 50.00; 16.67, 33.33 and 50.00 and 16.67, 16.66 and 33.33 in groups I, II and III, respectively. PIVD treatment resulted in 100 per cent oestrus response in buffaloes. Administration of hCG during FTAI increased the conception rate when compared to PIVD treatment alone. But injection GnRH during FTAI resulted in higher conception rate than hCG group in postpartum buffaloes.