Methods: Thirty crossbred cows were allocated into three groups, Group I with an ovsynch protocol: Group II with an additional GnRH on day 6 after 2nd GnRH of Ovsynch and Group III with an additional GnRH on day 12 after 2nd GnRH of Ovsynch. The average number of services required per conception for Group I, Group II and Group III was found 2.40, 2.00 and 2.20 respectively and the conception rate was 50.0, 60.0 and 50.00 respectively.

Result: The present study concluded that significantly (p<0.05) higher conception rate was recorded in Group II (60.00 per cent) than that observed (50.00) with Group I and Group III.

In conclusion, conception rate was better with an additional GnRH subsequent to ovsynch protocol with fixed time AI which can improve fertility response in crossbred cows.