Vol. 7, Issue 2, Part A (2022)

Author(s): IM Hassan, B Sa’idu, A Dahiru, SY Abiodun and SA Sofiat

Abstract: The study investigated the protective effect of methanolic leaf extracts of Solanum incanum in copper-induced nephrotoxicity. Animals were divided into four groups: A Control (maintained on food and water only), B treated with 300 mg/kg S. incanum, C treated with 200 mg/kg CuO_, and D treated with 300 mg/kg and then 200 mg/kg CuO. Serum electrolytes (sodium, potassium, chloride, bicarbonate) and kidney functional tests (urea, creatinine, and erythropoietin) were evaluated using enzyme-linked immunosorbent assay (ELISA). Prepared slides from the kidney were dehydrated using a dry air oven in xylene for 30 minutes and later mounted on the microscope and viewed using oil immersion ×1000 magnification. The result of electrolytes assays showed a significant decrease (P<0.05) with sodium, chloride, and bicarbonate concentration between the control groups 137.00 ± 4, 100.00 ± 3.46, 9.33 ± 3.18 compared with the group exposed to CuO only 93152.00 ± 1.00, 110.00 ± 2.04, 21.33 ± 0.88 (mmoL/L). The kidney function test shows no significant difference between the control group and the group treated with S. incanum methanol extract and then CuO. For the histopathological study, there were mild renal tubular degeneration and tubular dilation blue dot in groups exposed to CuO only. Because of the presence of a significant protective effect of electrolytes and as well kidney function, S. incanum is a potential source of remedy against CuO poisoning.

DOI: 10.22271/veterinary.2022.v7.i2a.413