Vol. 6, Issue 5, Part A (2021)

Author(s): Aremu Abdulfatai, Akorede Farhan Rhido, Akorede Ganiu Jimoh, Olatunji Omobolanle Aishat, Ahmed Olayiwola Akeem, Afisu Basiru and Suleiman Kolawole Yusuf

Abstract: Trypanosomosis is a disease complex affecting both human and animals. Chemotherapeutic approach to trypanosomosis is no longer befitting due to side effects associated with drugs and development of many resistant trypanosome worldwide. This study evaluated the polyherbal trypanocidal activities of Azadiractha indica and Lawsonia inermis when combined with diaminazene aceturate. Forty-five Wistar rats were randomly divided into nine groups. Groups: 1 (control), 2 (infected untreated), 3 (Diminazene), 4 (Lawsonia inermis (LI)), 5 (Azadiractha indica (AI)), 6 (LI+AI), 7 (LI+DA), 8 (LI+AI) and 9 (DA+LI+AI). All rats in groups (2-9) were infected with 3x10⁶ of Trypanosoma brucei per ml of blood. Result showed that both methanolic extract of Azadiractha indica and Lawsonia inermis contains important phytochemicals. Increased weight was observed in rats dosed with L. inermis+diminazene combination (6.3%) while A. indica treated rats presented significant (P<0.05) decreased weight. Survivability rate increased in most treated groups without noticeable relapse while 60% mortality was seen in group 8. Parasites were cleared within shortest time in the group treated with three combinations when compared to all other treatment groups. Haematological parameters were not significant (P>0.05) in most treatment groups compared to untreated control. This study concluded that both extracts reduced the level of parasitaemia in a transient phase and drug-extract combination cleared the parasitaemia within shortest time.

DOI: 10.22271/veterinary.2021.v6.i5a.384