Vol. 5, Issue 2, Part A (2020)

Author(s): Hlaing MinOo, Parawee Kanjanaprachoat, Tipwan Suppasat and Siriwat Wongsiri

Abstract: Thailand beekeeping has faced two main mites infections, there are Tropilaelaps mercedesae and Varroa destructor mites. They are very important for honeybee health and one of the economic losses factors of beekeeping in Thailand. In this study, we studied virus detection on the Tropilaelaps mercedesae and Varroa destructor by using the RT- PCR in Chiang Mai. Thirty Tropilaelaps mercedesae and Varroa destructor samples were detected with seven honeybee virus such as Deform Wing Virus (DWV), Acute Bee Paralysis Virus (ABPV), Chronic Bee Paralysis Virus (CBPV), Varroa Destructor Virus-1 (VDV-1), Sacbrood Virus (SBV), Kashmir Bee Virus (KBV) and Black Queen Cell Bee Virus (BQCV). We found that Tropilaelaps mercedesae have infected four honeybee viruses. Such as DWV, ABPV, CBPV and VDV-1. At the Varroa destructor, there have three honeybee viruses infected. Such as DWV, ABPV, and VDV-1. The percentage detection of DWV and ABPV are 100% detected and VDV-1 is 77 in Varroa destructor. And the Tropilaelaps mercedesae have DWV is 100% infected, ABPV is 57% infected, CBPV is 30% infected and VDV-1 is 23% detected. When compared the virus infection between the Tropilaelaps mercedesae and Varroa destructor mites, Tropilaelaps mercedesae have more virus infection than Varroa destructor.