Vol. 3, Issue 5, Part B (2018)

Author(s): MD Mehmood, Huma Anwar, Sana Noreen, Saud-ul-Hassan, Salman Ahmad and KA Mirza

Abstract: Antibody production in chicken is comparatively beneficial with respect to easy availability of birds and their management, relaxed handling in natural host. The study was attempted to raise polyclonal antibodies in broilers to examine their diagnostic and therapeutic potency simultaneously against avian Influenza virus subtypes and Newcastle disease virus. Healthy broilers were divided into 4 groups A, B, C and D. Birds in group A, B, C and D showed 1024, 512, 1024 and 1024 peak anti-influenza H5N1-HI antibody, anti-influenza H7N3-HI antibody, anti-influenza H9N2-HI antibody and anti NDV-HI antibody titer respectively on 42 day post vaccination. Whereas, therapeutic studies indicates 100% protection in naturally infected laboratory confirmed NDV commercial broilers in contrast to AIV H9N2 infected broilers where 80% protection is achieved. Polyclonal antiserum can be used for large-scale screening of AI and NDV carrier commercial and wild birds. Moreover, current study suggests that purified polyclonal antiserum could be used in successful treatment of avian Influenza virus infected broilers.