Vol. 2, Issue 1, Part A (2017)

Author(s): R Gogoi, DC Roy, S Sarma, RK Roy and SK Laskar

Abstract: The present study was conducted to investigate residues of Ciprofloxacin in raw milk of cow. A total of 175 raw milk samples of cow were collected from different districts of Assam. Ultra High Performance Liquid Chromatography (UHPLC) with diode array detection system was employed for detection of ciprofloxacin in the milk samples. A mixture of water and acetonitrile (70:30 v/v) was used as the mobile phase. The flow rate was maintained in an isocratic mode at a speed of 1.0 ml.min-1. The wavelength for the detector was set at 277 nm. A standard calibration curve with coefficient of determination of 99.64%. The method yielded good recovery percentage of ciprofloxacin in milk which ranged from 94-99%. Out of the 175 screened samples, 0.57 % sample was detected to be positive for residues of Ciprofloxacin.